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31.
V A Brumberg L Z Pevzner T E Poroshina 《Biulleten' eksperimental'no? biologii i meditsiny》1975,80(10):60-62
It was shown spectrophotometrically that in Krushinsky-Molodkina and Wistar rats the ratio of the activity of the aerobic H-forms of lactic dehydrogenase (LDH) to the activity of the anaerobic M-forms was higher in the neurons of the cerebral cortex and the Purkinje's cells of the cerebellum and in their glial cells-satellites than in the motor neurons of the anterior horns of the spinal cord and their perineuronal glia. In Krushinsky-Molodkina rats (with genetically-determined high sensitivity to audiogenic convulsions) epileptiform attacks under the effect of sound were accompanied by a marked activation of both the H- and the M-forms of LDH in the cortical neurons in the absence of any shifts in the perineuronal glia. On the contrary, the activity of all the forms of LDH was unchanged in the spinal motor neurons, whereas in the neuroglia cells surrounding these neurons there was a distinct increase in the activity of the H-forms of LDH. In the Purkinje's cells of the cerebellum an increase and in the glial cells- satellites -- a reduction of the activity of the M-forms of LDH in case of convulsions was seen. 相似文献
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Mutation-tolerant protein identification by mass spectrometry. 总被引:8,自引:0,他引:8
Database search in tandem mass spectrometry is a powerful tool for protein identification. High-throughput spectral acquisition raises the problem of dealing with genetic variation and peptide modifications within a population of related proteins. A method that cross-correlates and clusters related spectra in large collections of uncharacterized spectra (i.e., from normal and diseased individuals) would be very valuable in functional proteomics. This problem is far from being simple since very similar peptides may have very different spectra. We introduce a new notion of spectral similarity that allows one to identify related spectra even if the corresponding peptides have multiple modifications/mutations. Based on this notion, we developed a new algorithm for mutation-tolerant database search as well as a method for cross-correlating related uncharacterized spectra. 相似文献
36.
A study of bacterial surface oligosaccharides were investigated among
different strains of Neisseria gonorrhoeae to correlate structural features
essential for binding to the MAb 2C7. This epitope is widely expressed and
conserved in gonococcal isolates, characteristics essential to an effective
candidate vaccine antigen. Sample lipooligosaccharides (LOS), was prepared
by a modification of the hot phenol-water method from which de-O-acetylated
LOS and oligosaccharide (OS) components were analyzed by ES-MS-CID-MS and
ES-MSnin a triple quadrupole and an ion trap mass spectrometer,
respectively. Previously documented natural heterogeneity was apparent from
both LOS and OS preparations which was admixed with fragments induced by
hydrazine and mild acid treatment. Natural heterogeneity was limited to
phosphorylation and antenni extensions to the alpha-chain. Mild acid
hydrolysis to release OS also hydrolyzed the beta(1-->6) glycosidic
linkage of lipid A. OS structures were determined by collisional and
resonance excitation combined with MS and multistep MSn which provided
sequence information from both neutral loss, and nonreducing terminal
fragments. A comparison of OS structures, with earlier knowledge of MAb
binding, enzyme treatment, and partial acid hydrolysis indicates a generic
overlapping domain for 2C7 binding. Reoccurring structural features include
a Hepalpha(1-->3)Hepbeta(1-->5)KDO trisaccharide core branched on the
nonreducing terminus (Hep-2) with an alpha(1-->2) linked GlcNAc
(gamma-chain), and an alpha-linked lactose (beta-chain) residue. From the
central heptose (Hep-1), a beta(1-->4) linked lactose (alpha-chain),
moiety is required although extensions to this residue appear unnecessary.
相似文献
37.
Kristin E. Burnum-Johnson Thomas P. Conrads Richard R. Drake Amy E. Herr Ravi Iyengar Ryan T. Kelly Emma Lundberg Michael J. MacCoss Alexandra Naba Garry P. Nolan Pavel A. Pevzner Karin D. Rodland Salvatore Sechi Nikolai Slavov Jeffrey M. Spraggins Jennifer E. Van Eyk Marc Vidal Christine Vogel Neil L. Kelleher 《Molecular & cellular proteomics : MCP》2022,21(7):100254
All human diseases involve proteins, yet our current tools to characterize and quantify them are limited. To better elucidate proteins across space, time, and molecular composition, we provide a >10 years of projection for technologies to meet the challenges that protein biology presents. With a broad perspective, we discuss grand opportunities to transition the science of proteomics into a more propulsive enterprise. Extrapolating recent trends, we describe a next generation of approaches to define, quantify, and visualize the multiple dimensions of the proteome, thereby transforming our understanding and interactions with human disease in the coming decade. 相似文献
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Upon searching local similarities in long sequences, the necessityof a rapid similarity search becomes acute. Quadraticcomplexity of dynamic programming algorithms forces the employmentof filtration methods that allow elimination of the sequenceswith a low similarity level. The paper is devoted to the theoreticalsubstantiations of the filtration method based on the statisticaldistance between texts. The notion of the filtration efficiencyis introduced and the efficiency of several filters is estimated.It is shown that the efficiency of the statistical l-tuple filtrationupon DNA database search is associated with a potential extensionof the original fourletter alphabet and grows exponentiallywith increasing l. The formula that allows one to estimate thefiltration parameters is presented. 相似文献
40.
L. D. Zorova V. A. Popkov E. J. Plotnikov D. N. Silachev I. B. Pevzner S. S. Jankauskas S. D. Zorov V. A. Babenko D. B. Zorov 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2018,12(1):20-26
The electrical polarization of the inner mitochondrial membrane largely determines the electrochemical potential of hydrogen ifons, being thereby a significant factor in the energy transformation during oxidation of respiratory substrates and its accumulation in the form of newly synthesized ATP. However, the gradient of the electric potential on the inner mitochondrial membrane (ΔΨm) performs a number of functions not related to energy production. Even under hypoxic conditions, precluding the formation of ATP in mitochondria through oxidative phosphorylation, mitochondria maintain their ΔΨm at the expense of the hydrolysis of cellular ATP, which indicates the exceptional importance of ΔΨm for non-energetic functions of mitochondria. Among these functions, the mitochondrial inward transport of metal cations and proteins carrying a positively charged amino acid sequence and export of anions including nucleic acids possibly providing retrograde signaling, seem very important and essential for maintaining mitochondrial structure and metabolism. ΔΨm is a powerful regulator of mitochondrial generation of reactive oxygen species that perform physiological and pathological functions. And finally, ΔΨm is a critical element in the mechanism of disposal of dysfunctional mitochondria, the so-called quality control machinery of mitochondria. The disturbance of this mechanism leads to increase of heterogeneity in the population of mitochondria in the cell, and the degree of heterogeneity can be considered as an indicator of the pathological cellular phenotype. Correlation between Ψm and cell functions is difficult to identify without adequate quantitative estimates of the magnitude of ΔΨm, which are complicated due to several cellular and mitochondrial processes that affect the experimentally obtained values. Recommendations for assessing the contribution of these processes and avoiding artifacts in the measurements of ΔΨm by standard methods are given. 相似文献